Anti-6X His tag® antibody [3D5] – C-terminal
Monoclonal rat [3D5] at 6X His tag® – C-terminal
Host species: Rat
ab206505 reacts with recombinant proteins containing C-terminal 6X His-tagged recombinant fused to the carboxy terminus of the fusion proteins. It also has an affinity for the internal 6X His tag® but no affinity for the 6X His tag® fused to the amino terminus.
Suitable for: WB, ELISA
Reacts With: Independent Species
Recombinant fragment corresponding to 6X His tag®. Purified His-tagged Fab fragment.
ab206505 is a chimeric antibody created to improve compatibility with existing reagents, assays and techniques.
Shipped at 4°C. Store at +4°C for the short term (1-2 weeks). At the time of aliquot delivery. Store at -20°C long term. Avoid freeze/thaw cycle.
- Preservative: 0.02% Proclin 300
- Constituent: 99% PBS
Purity: purified protein A
Clone number: 3D5
Light chain type: kappa
The Abpromise Guarantee
Our Abpromise warranty covers the use of ab206505 in the following tested applications. Application notes include recommended starting dilutions; the end-user must determine the optimal dilutions/concentrations.
The H-H-H-H-H-H motif is used as a tag in many recombinant proteins to facilitate purification. His tags can be fused to the amino or carboxy termini of proteins in transfected or transformed cells.
It depends on the location of the original hexahistidine-tagged protein.
- 6 His epitope tag antibody
- Hexa-his tag antibody
- HHHHHH epitope tag antibody